Biophysics, Poster

EFFECT OF GLYCATION ON THE GLUCOSE DIFFUSIVITY IN SKIN TISSUE: MODEL STUDIES WITH MOUSE SKIN

Daria K.Tuchina,1,2 Rui Shi,1 Alexey N. Bashkatov,2 Elina A. Genina,2 Dan Zhu,1 Qingming Luo,1 Valery V. Tuchin1-4

1Britton Chance Center for Biomedical Photonics,Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China
2Research-Educational Institute of Optics and Biophotonics, Saratov State University, Saratov, 410012, Russia
3Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precise Mechanics and Control RAS, Saratov, 410028, Russia
4Optoelectronics and Measurement Techniques Laboratory, P.O. Box 4500, University of Oulu, FIN-90014, Oulu, Finland

ABSTRACT

We present results on determination of glucose diffusion coefficients in skin of healthy mice and mice with diabetes in vitro. The method is based on the measurement of optical collimated transmittance kinetics of the tissue concurrently with administration of the chemical agent.
Ten skin samples from healthy mice and ten skin samples from diabetic mice were measured concurrently with administration of aqueous 30%-glucose solution. The transmittance spectra have been measured using USB4000-Vis-NIR spectrometer (Ocean Optics, USA) in the spectral range 400-1000 nm. The measurements were performed at room temperature about 20°C. Diabetes was induced by subcutaneous injection of alloxan to animals.
Increase of the collimated transmittance of skin samples of healthy and diabetic mice was observed. Glucose diffusion coefficients were estimated as (2.71±1.09х10-6 cm2/sec in skin of healthy mice and (1.24±0.51)х10-6 cm2/sec in skin of mice with diabetes. These results can be used for optimization of optical clearing technologies and for improvement of diagnostics and monitoring of diabetes mellitus.

Representing author

photo

Daria Kirillovna Tuchina

Saratov National Research State University, Assistant Professor, Engineer
Saratov, Russia

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