Daria K.Tuchina1, Alexey N. Bashkatov1, Elina A. Genina1, Vyacheslav I. Kochubey1, Valery V. Tuchin1-3
1Research-Educational Institute of Optics and Biophotonics, Saratov State University, Saratov, 410012, Russia
2 Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precise Mechanics and Control RAS, Saratov, 410028, Russia
3Optoelectronics and Measurement Techniques Laboratory, P.O. Box 4500, University of Oulu, FIN-90014, Oulu, Finland
We present results of in vitro skin optical clearing by 40%-glucose solution. Ten samples of intact rat skin were measured to obtain spectra of skin optical properties before and after incubation in 40%-glucose solution. Diffuse reflectance and total and collimated transmittance spectra were measured by LAMBDA 950 (Perkin Elmer, USA) spectrophotometer with an integrating sphere in the spectral range 350 – 2500 nm. All measurements have been performed during 24 hours after obtaining the samples. Then the samples were incubated during 24 hours in 40%-glucose solution and all of the spectra were measured again. The last stage was incubating the samples during 24 hours in physiological solution and measuring spectra after that. Inverse Monte Carlo technique has been used for processing the experimentally measured spectra of the skin samples; and wavelength dependence of absorption and scattering coefficients, and anisotropy factor has been obtained.
Glucose diffusion coefficient was estimated from the measurement of collimated transmittance. The collimated transmittance was measured in the spectral range 400-1000 nm for ten rat skin samples using USB4000 spectrometer (Ocean Optics, USA) concurrently with administration of 40%-glucose solution and diffusion coefficient was evaluated using a specially developed computer program. The average value of glucose diffusion coefficient was estimated as (1.52±1.62)´106 cm2/sec. The presented results can be useful for the development of the optical imaging technologies and diagnostics and therapy of diabetes mellitus.
Daria Kirillovna Tuchina
Saratov National Research State University, Assistant Professor, Engineer
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