Nanostructures, Oral Report

STUDY OF LIPOPROTEINS AND ARTERIAL INTIMA INTERACTION BASED ON ARTERIAL ENDOTHELIAL CELLS REAL GEOMETRICAL STRUCTURE

О.Е. Глухова, д-р физ.-мат. наук, профессор (O.E. Glukhova)
И.В. Кириллова, канд. физ.-мат наук, доцент, директор образовательно-научного института наноструктур и биосистем (I.V. Kirillova)
Г.Н. Маслякова*, д-р мед. наук, профессор, зав. каф. патологической анатомии (G.N. Maslyakova)
Е.Л. Коссович, научный сотрудник (E.L. Kossovich)
Д.А. Заярский, ведущий инженер (D.A. Zayarsky)
А.А. Фадеев, старший лаборант (A.A. Fadeev)

Саратовский государственный университет имени Н.Г. Чернышевского
* Саратовский государственный медицинский университет им. Н.В. Разумовского

ABSTRACT

Nowadays cardiovascular system diseases take the first place in the Russian Federation popularity mortality factors. The direct cause of the cardiovascular pathology development is found to be the atherosclerosis plaques formation inside the vessel walls. This process leads to development of atherosclerosis. Atherosclerosis is a chronic disease which is based on formation of lipoprotein (cholesterol) spots and strips. The formation is based on low density lipoproteins penetration into the vessels intima.
In the work we developed a new scanning technique for arterial intima morphology using the atomic force microscopy and sonde-type nanolaboratory NTEGRASpectra (NT-MDT, Russian Federation). As a result, we obtained the topology of human coronary artery intima with a nanometer resolution. The 3D model for the “endothelial cell surface – low density lipoprotein (LDL)” was constructed. We utilized this model in order to calculate the LDL deformation rate using the ANSYS finite element modeling software package. It also allowed us to find the stress distribution at the moment of the falling LDL interaction with the endothelial cell surface. The largest normal and tangential stresses are observed in the area of the LDL and endothelial surface interaction. The values are 2,173 and 0,053 kPa, respectively. It was also shown that the LDL structure is being deformed after such an impact. This could lead to the macromolecule compression and wreck. Therefore, when the LDL hits the intercellular space between the endothelial cells, the macromolecule can be compressed and its atomic structure can be altered.

Representing author

photo

Aleksandr Andreevich Fadeev

SGU, Студент
Саратов, Russia

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