RCW'12, Poster

IN VITRO OCT STUDY OF ALTERATIONS OF ADIPOSE TISSUE STRUCTURE INDUCED BY PDT TREATMENT

Irina Yu. Yanina,1,2 Natalia A. Trunina,1 Valery V. Tuchin,1,3,4
1Saratov State University (SGU), Russia
2Saratov State Medical University (SGMU), Russia
3Institute of Precise Mechanics and Control RAS, Russia
4University of Oulu, Finland

ABSTRACT

Temporal changes in structure and refractive index distribution of adipose tissue at photodynamic treatment were studied with OCT. The 100-150 m fat tissues slices were used in in vitro experiments. Water-ethanol solutions of indocyanine green (ICG) and brilliant green (BG) of 1 mg/ml and 6 mg/ml concentration, respectively, were used for fat tissue staining. CW laser diode (ACCULASER, 810 nm) and dental diode irradiator Ultra Lume Led 5 (442 and 597 nm) were used for irradiation of tissue slices. Laser irradiation time was 1 min, and the diode lamp 5 min. Power densities were 250 and 75 mW/cm2, respectively. The studies were conducted at room temperature.
It was found that relative refractive index of the scatterers decreased with time elapsed after treatment that indicated the immersion optical clearing. These data support the hypothesis that photodynamic treatment induces fat cell lipolysis for some period after treatment.

Representing author

photo

Irina Yur'evna Yanina

Saratov State University, scientific researcher
Saratov, Russia

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