Biophotonics, Internet Report

OPTICAL MEASUREMENTS OF RAT MUSCLE SAMPLES UNDER TREATMENT WITH ETHYLENE GLYCOL AND GLUCOSE

Luis Oliveira, DFI – Polytechnic of Porto, School of Engineering, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal; PhD student at FEUP - University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal

Maria Inês Carvalho, DEEC/FEUP and INESC TEC, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal

Elisabete Nogueira, DFI – Polytechnic of Porto, School of Engineering, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal

Valery V. Tuchin, Research-Educational Institute of Optics and Biophotonics, Saratov State University, 83 Astrakhanskaya str., Saratov 410012, Russia; Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precise Mechanics and Control RAS, Saratov, 410028, Russia; Optoelectronics and Measurement Techniques Laboratory, P.O. Box 4500, University of Oulu, FIN-90014, Oulu, Finland

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ABSTRACT

With the objective to study the variation of optical properties of rat muscle during optical clearing, we have performed a set of optical measurements from that kind of tissue. The measurements performed were total transmittance (Tt), collimated transmittance (Tc), specular reflectance (Rs) and total reflectance (Rt). This set of measurements is sufficient to determine diffuse reflectance and absorbance of the sample. All these measurements and calculated quantities will be used later in inverse Monte Carlo (IMC) simulations to determine the evolution of the optical properties of muscle during treatments with ethylene glycol and glucose. The results obtained with the measurements already provide some information about the optical clearing treatments applied to the muscle and translate the mechanisms of turning the tissue more transparent and sequence of regimes of optical clearing.

Representing author

photo

Prof. Luis Manuel Oliveira

Instituto Superior de Engenharia do Porto, Professor
Porto, Portugal

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