Xiaoqin Zhu, Fujian Normal University, China
Jianxin Chen,Fujian Normal University, China
Abstract: Collagen deposition plays an important role in elastic cartilage repair. Label-free imaging and characterizing the collagen of repairing cartilages is critical toward understanding the mechanism of cartilage repair and efficacy of potential therapeutics. In this study, elastic cartilage repair in a rabbit ear model was examined with second-harmonic generation (SHG) microscopy without using labeling agents. High resolution SHG images of repairing tissues were obtained and image texture was quantitatively analyzed showing significant alteration in collagen content and distribution associated with cartilage repair. Our results suggested that SHG microscopy has the potential in label-freely imaging the repairing cartilage dynamic with time, thus monitoring the cartilage repair progression at a molecular level, and guiding the establishment of novel efficacious therapeutic strategies based on the better understanding of response mechanism.
Key words: second-harmonic generation microscopy, elastic cartilage repair, collagen
Dr. Xiaoqin Zhu
Fujian Normal University
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