Internet Biophotonics XI, Internet Invited Lecture

INVESTIGATION OF THE REVERSIBLE PERMEABILITY OF NEURON MEMBRANE INDUCED BY TERAHERTZ RADIATION

A. S. Ratushniak, Institute of Computational Technologies of SB RAS, Novosibirsk, 630090 Russia
T. A. Zapara, Institute of Computational Technologies of SB RAS, Novosibirsk, 630090 Russia
A. L. Proskura, Institute of Computational Technologies of SB RAS, Novosibirsk, 630090 Russia
A.S. Kozlov, Institute of Chemical Kinetics and Combustion of SB RAS, Novosibirsk, 630090 Russia
D.S. Serdyukov, Institute of Laser Physics of SB RAS, Novosibirsk, 630090 Russia
Novosibirsk National Research State University, Novosibirsk, 630090 Russia
Federal Research Center "Institute of Cytology and Genetics of the Siberian Branch of the RAS", Novosibirsk, 630090 Russia
Olga Cherkasova, Institute of Laser Physics SB RAS, Novosibirsk, 630090 Russia
Novosibirsk State Technical University, Novosibirsk, 630073 Russia

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ABSTRACT

A number of review articles have shown that THz radiation affects the conformation and function of proteins and nucleic acids and influences the penetrability of cell membranes and the properties of cells [1, 2]. The aim of the work is to study the mechanisms of THz radiation influence on membranes and neural signalling pathways, which alters intermolecular interactions, including the permeability of cell membranes.
The study was carried out using neurons of the molluscan Lymnaea stagnalis, isolated and cultured in vitro. Larger size of neurons and good level of scrutiny of biophysical properties allow carrying out multiphasic screening of possible effects. We have demonstrated that some wavelengths of THz range have a highly specific influence on morphology of isolated neurons at the network neogenesis stage under normal conditions [3]. We have shown that THz radiation with the wavelength of 130 mkm provides the conditions for the penetration of vital dyes into the neural cells [4].
The functional characteristics of the cells were not changed. To verify the assumptions concerning the mechanisms of the discovered phenomenon, in addition to the dye (Lucifer yellow) which does not penetrate through intact membranes, antioxidants were introduced before irradiation into the saline solution surrounding the neurons. It was discovered that the phenolic antioxidant histochrome causes a substantial decrease in the penetration of the dye into the cell. A decrease in the level of dye penetration into neurons allowed us to assume that under the action of THz radiation, hydrophilic pores arise in the cell membranes as a result of free radical processes that can block by antioxidants. It was established that THz radiation can cause reversible distortions of the barrier properties of membranes while antioxidants may act as the modulators of this process and serve as a protection from the unfavorable action of electromagnetic waves in this range.
Reference:
[1] V.I. Fedorov, D.S.Serdyukov, O.P.Cherkasova, et al, Journal of Optical Technology 84 (8), 509-514 (2017) , doi 10.1364/JOT.84.000509.
[2] S.Romanenko, R.Begley, A.R.Harvey, L.Hool, V.P.Wallace, J R Soc Interface 14 (2017), doi: 10.1098/rsif.2017.0585.
[3] J.S.Olshevskaya,A.S.Ratushnyak, A.K.Petrov, A.S.Kozlov, T.A. Zapara, Proceedings 2008 IEEE Region 8 international conference on computational technologies in electrical and electronics engineering “SIBIRCON 2008”, 210-211 (2008), doi: 10.1109/SIBIRCON.2008.4602607.
[4] T. A. Zapara, S. P. Treskova, A. S. Ratushniak, Journal of Surface Investigation. X-ray, Synchrotron and Neutron Techniques 9 (5), 869–871 (2015).

Representing author

photo

Prof. Olga Pavlovna Cherkasova

Institute of Laser Physics SB RAS, Novosibirsk State Technical University, Head of Biophysics Laboratory
Novosibirsk, Russia

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