E. Belina(1), H. Kisov(1), G. Dyankov(1), E. Borisova(2,3), E. Pavlova(4)
1) Institute of Optical Materials and Technologies, 109 Acad. G. Bonchev str., 1113 Sofia, Bulgaria
2) Institute of Electronics, Bulgarian Academy of Sciences, 72 Tsarigradsko chaussee Blvd., 1784 Sofia, Bulgaria
3) Biology Department, Saratov State University, 83, Astrakhanskaya Str., 410012 Saratov, Russia
4) Faculty of Physics, Sofia University “St. Kl. Ohridski” Sofia, 5 James Bouchier Blvd.,
The immobilization of the biorecognized elements on the transducer is the key factor in developing a reliable biosensor. Hundreds of immobilization protocols have been developed in an effort to solve the problem. All of them are focused on finding appropriate matrices in which the recognition substance can be incorporated. Depending on the type of transducer (electrical, optical and etc.) the matrix must provide corresponding properties for effective performance. For example, in the case of SPR transducer good adhesion and a layer with fixed thickness have to be achieved. However, the matrix always deteriorates the effectiveness of recognition. The best approach is to perform direct immobilization of the recognition substance.
Here we report for elaboration of the first, to best of our knowledge, SPR biochip with immobilized myoglobin and hemoglobin. The direct immobilization was performed by spin coating. Also, the matrix-assisted pulsed-laser evaporation (MAPLE) was used for immobilization. The bioactivity of elaborated biochips was studied by registration of binding activity of CO, NO and CO2 to the myoglobin and hemoglobin. We show that the bioactivity of direct immobilized layers is much higher than that created by MAPLE, however the durability is worse.
Acknowledgements: This work is supported by National Science Fund of Ministry of Education and Sciences in the frames of project #DN18/8/2017 “Biochip, based on new plasmon structures and nanostructured bio-sensing elements”.
Dr. Ekaterina Georgieva Borisova
Institute of Electronics, Bulgarian Academy of Sciences, Associate Professor
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