Nanobiophotonics, Poster

POLYETHYLENEIMINE-ENTRAPPED GOLD NANOPARTICLES AS A POTENTIAL DELIVERY SYSTEM FOR DNA VACCINES AGAINST AFRICAN SWINE FEVER VIRUS

Timofey Pylaev, IBPPM RAS, Russia
Alexander Fomin, IBPPM RAS, Saratov, Russia, Saratov Scientific and Research Veterinary Institute, Russian Academy of Agricultural Sciences, Saratov, Russia,
Ekaterina Vanzha, IBPPM RAS, Saratov, Russia
Sergey Staroverov, IBPPM RAS, Saratov, Russia, Saratov Scientific and Research Veterinary Institute, Russian Academy of Agricultural Sciences, Saratov, Russia,
Nikolai Khlebtsov, IBPPM RAS, Saratov, Russia

ABSTRACT

The need to develop new drugs and approaches to the prevention of infectious diseases in livestock breeding is dictated by issues related to the growing need for food security and food independence of Russia. One of the approaches to fight with infectious diseases is DNA vaccination. The method of gene immunization, often called DNA vaccination, is developed in experiments with animals, showed high efficacy, especially with respect to viral infections - tick-borne encephalitis, HIV infection, hepatitis B and
some others. Delivery systems based on micro- and nanoparticles that encapsulate or adsorb plasmid DNA are the most promising DNA vaccines delivering strategies. Such systems ensure the passive entry of genetic material into antigen-presenting cells. One of the most popular DNA-carriers nanomaterials to the date are colloidal gold nanoparticles (GNPs). GNPs are
promising not only for diagnostic purposes, but also for nucleic acids delivery, due to their biologically inertness and low-toxicity. African swine fever (ASF) is a highly contagious viral disease of pigs, characterized by extremely high
mortality of infected animals. In connection with the current situation, it is urgent to develop vaccines to fight this disease. In the known literature there is no information on the development of prototype DNA vaccines against African swine fever and the mechanism of delivery of immunogenic structures using gold nanoparticles. The present study is aimed to evaluate the prospects of using gold nanoparticles as a carrier of genetic information into eukaryotic cells along with immunization of animals and the development of DNA vaccines prototypes against African swine fever (ASF).

Representing author

photo

Dr. Timofey Evgen'evich Pylaev

IBPPM RAS, PhD, Researcher in Lab of Nanobiotechnology, IBPPM RAS
Saratov, Russia

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