Biophotonics, Internet Plenary Lecture

IN VIVO SKIN OPTICAL CLEARING WINDOW FOR CUTANEOUS VASCULAR AND CELL IMAGING

Dan Zhu (1,2), (1) Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; (2) MOE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China

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ABSTRACT

The optical technology has provided significant tool for molecular imaging and medical diagnosis with high sensitivity and resolution. However, the high scattering of turbid skin limits the penetration of visible and near-infrared light in tissue, which affects its applicability in preclinical and clinical medicine, and other life science. The tissue optical clearing technique based on immersion of tissues into optical clearing agents (OCAs) with high refractive indices, hyperosmolarity and biocompatibility allows one to effectively control optical properties of tissues, leads to essential reduction of scattering, therefore, enhances the depth to which light penetrates in tissue, and currently attracts great attentions. This technique, combined with various optical techniques has shown a great potential for improving the capabilities of optical imaging, diagnosis and therapeutic treatments. During the past years, we developed various in vivo skin optical clearing methods, from mice dorsal skin, ear skin to footpad skin, which not only have the repeatability, but also have the reversibility and safety, just like a switchable window. The skin optical clearing window allows us to image cutaneous vascular and cell function, such as blood flow, blood oxygen, vascular structure and cell movement by using laser speckle imaging, hyperspectral imaging, Angio-OCT, in vivo flow cytometer and fluorescence imaging, etc.

Representing author

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Dr. Dan Zhu

Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology, Professor
Wuhan, P.R.China

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