Biophysics, Plenary Lecture

MULTIPARAMETRIC ANALYSIS OF TUMOR DEVELOPMENT AND RESPONSE FOR CHEMOTHERAPY USING TIME-RESOLVED IMAGING

Elena Zagaynova, Nizhny Novgorod State Medical Academy, Russia
Marina Shirmanova, Nizhny Novgorod State Medical Academy, Russia
Marina Kuimova, Imperial College, London
Konstantin Lukyanov, Institute of Bioorganic chemistry, Russia
Maria Lukina, Nizhny Novgorod State Medical Academy, Russia
Lubov Shimolina, Nizhny Novgorod State Medical Academy, Russia
Varvara Dudenkova, Nizhny Novgorod State Medical Academy, Russia
Tatyana Sergeeva, Nizhny Novgorod State Medical Academy, Russia
Natalia Klementieva, Nizhny Novgorod State Medical Academy, Russia
Vladislav Scheslavsky, Becker & Hickl GmbH, Berlin, Germany
Irina Druzhkova, Nizhny Novgorod State Medical Academy, Russia

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ABSTRACT

The present work demonstrates the use of Fluorescent imaging, including FLIM/PLIM, to monitor metabolic and oxygen state, microviscosity, cytoskeleton changes, proliferative activity of cancer cells during tumor development and response to chemotherapy. Analysis of cellular metabolism was based on the visualization of intrinsic fluorescent cofactors NAD(P)H and FAD. Microscopic viscosity of plasma membrane was measured using fluorescent molecular rotor and FLIM. Proliferative capacity of cells was assessed using novel FUCCI-based cell cycle indicator and FLIM. Cytoplasmic pH was measured using genetically encoded sensor SypHer2. Cytoskeleton changes were estimated by actin-associated fluorescent protein and STORM microscopy. Apoptosis during chemotherapy was verified by Caspase FRET-based sensor. And also time resolved imaging was used to simultaneously measured of metabolic and oxygen state in tumor.

Representing author

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Prof. Elena Vadimovna Zagaynova

Nizhny Novgorod State Medical Academy, Director of Institute of Biomedical Technologies
Nizhny Novgorod, Russia

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