V.A. Doubrovski, Yu. A. Ganilova, I.V. Zabenkov, A.A. Dolmashkin
Saratov State Medical University named after V.I. Razumovski, Russia
On the base of digital flow microscopy large immune RBC complexes (agglutinates) were revealed experimentally as red while free erythrocytes and small agglutinates were dark-green when the fluid flow of mixture solution “blood – serum” was illuminated by spectrally filtered light beam with maximum in green range. This phenomenon was used to register the erythrocytes agglutination reaction – the base of blood group typing for human beings. On the base of spectral measurements for blood samples and subsequent calculations of spectral characteristics of light absorption and scattering coefficients minimum size of erythrocytic formation was estimated when brightness of red component of RGB decomposition of the digital photo prevail over green one. Such a formation was red, it was assumed as an immune RBC complex. Spectrally filtered light beam application in combination with digital photo processing suggested here increases the reliability of immune RBC complexes registration, hence, human blood group typing.
Irina Yur'evna Yanina
Saratov State University, scientific researcher
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