Biophysics, Plenary Lecture

ADVANCED METHODS OF 3D LIVE CELL MICROSCOPY

Prof. Dr. Herbert Schneckenburger

Institute of Applied Research, Aalen University, Germany

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ABSTRACT

3D microscopy of living cells (including larger cell spheroids), tissue samples and small organisms. Here, it will be shown how information from various focal planes of a microscope can be obtained and combined in a 3D image. Relevant techniques include Confocal Laser Scanning Microscopy (CLSM), Structured Illumination Microscopy (SIM) and Light Sheet based Fluorescence Microscopy (LSFM). While LSFM permits the lowest light exposure and allows cells to be kept vital even over prolonged measuring procedures, SIM permits the highest resolution and – in combination with axial tomography – represents an important step towards super-resolution microscopy. Finally, it will be demonstrated how fluorescence measurements can support laser micromanipulation including laser tweezers and laser assisted optoporation.

Representing author

photo

Prof. Herbert Schneckenburger

Institut fur Angewandte Forschung Hochschule Aalen, ~
Aalen, Germany

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