Taskina L.A., Timchenko E.V., Timchenko P.E., Zaharov V.P., SSAU, Russia
The results of experimental research on shift of spectral characteristics of various types of plants under external influence were presented. Differential optical coefficient was used as a controlled optical parameter, which characterized the change in concentration of chlorophyll during exposure to external factors. The main optical controlling method was differential backscattered method, which is highly sensitive and provides the most accurate information about the condition of living system. For the understanding of mechanisms of accumulation of external factors on living systems, the confocal fluorescence microscopy method, which provides contrast high resolution micro photos, was used in the current study for microscopical analysis.
As a result of the conducted research functional dependencies of the optical coefficient from the intensity of external factors' influence were found. It was discovered, that with increasing of intensity of the environmental external factor (increase of the concentration of Synthetic Surfactants in water plants and nitrates in land plants) the most significant changes of plants optical characteristics have happened.
It was proven with microscopic study that the mechanism of the chlorophyll degradation under external influence is corresponding with solubilization of membrane proteins and changes in its permeability.
The method of confocal fluorescence microscopy revealed that the external factors containing the chemical elements included in the structural formula of chlorophyll, on the expiration of time result in a change in membrane organization of chloroplasts by increasing its size, which in turn is reflected in changes of chlorophyll fluorescence.
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