I.Yu. Yanina1, V.V. Tuchin1,2,3 ,
S.A. Portnov4, Yu.I. Svenskaya4, D. A. Gorin4
E.G. Ponomareva5, V.E. Nikitina5,
1Physics Department, Saratov State University, Saratov, Russia;
2Institute of Precise Mechanics and Control RAS, Russia;
3University of Oulu, Finland;
4Nano- and Biomedical Technology Department, Saratov State University, Saratov, Russia
5Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russian Federation
The influence of bacterial lectin on photodynamically induced fat cell lipolysis was studied. Lectin of concentration of 0.002 mg / ml, isolated from the strain of Azospirillum brasilense Sp7, was used. The 100-150 μm adipose tissues slices were used in in vitro experiments. A CW diode laser (ACCULASER, 810 nm) was used to irradiate tissue slices at power densities of 250, 375, 500, and 625 mW/cm2.
The 2 mg/ml ICG water solutions were encapsulated by a three-step procedure. First, CaCO3 particles filled with ICG were formed by co-precipitation of Na2CO3 and CaCl2 in ICG solution. Then polyelectrolyte shell was formed by layer-by-layer self-assembly method. Biocompatible cationic (chitozan) and anionic (dextran sulfate) polyelectrolytes were deposited from 1 mg/ml solutions in 0.15M NaCl. On the third step CaCO3 cores were dissolved by 2M EDTA water solution.
Resulting capsules were tested for ICG presence by optical absorption spectra measurements. To separate released and encapsulated ICG centrifugation was used following with supernatant removal and capsules resuspension in pure deionized water. Supernatant and capsules suspensions spectra were measured separately.
It was also found that pretreatment of tissue by lectin leads to 2-fold acceleration of lipolysis at photodynamic treatment. The data obtained can be used to enhance efficiency of photodynamic therapy.
Irina Yur'evna Yanina
Saratov State University, scientific researcher
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