Biophysics, Poster

STUDIES OF LIPID PEROXIDATION OF RAT BLOOD AFTER IN VIVO PHOTODYNAMIC TREATMENT

Irina Yu. Yanina,1,2 Nikita A. Navolokin,2 Cyan Myau,2
Alla B. Bucharskaya,2 Galina N. Мaslyakova,2 and Valery V. Tuchin,1,3,4
1Saratov State University, Russia
2Saratov State Medical University, Russia
3Institute of Precise Mechanics and Control RAS, Russia
3University of Oulu, Finland

ABSTRACT

Lipid peroxidation of blood serum of laboratory animals after in vivo photodynamic treatment was investigated. Group of 2-year old female rats of 10 animals kept 14 days in accordance with the European Convention for the Protection of animals used for experimental and other scientific purposes, besides the standard diet received 5 g sugar, 5 g dry milk, 5 g of sunflower oil and 5 g of egg powder in order to cause alimentary obesity. Animals were divided into 2 groups of 5 individuals. Hairs were removed. The action was carried out in area of the ribs on the side of the chest. The ICG in concentration of 0.5 mg / ml was injected into …... To the first group of rats injected ICG was dissolved in saline (solution 1), while to the second group - dissolved in a special mixture consisting of 25% alcohol, 25% glycerol and 50% distilled water (solution 2). Within rat ICG (solution 1 or 2 of the same concentration) was injected first, and then irradiated with a diode NIR-laser (wavelength, 808 nm; power density, 16-24 W/cm2; exposure time, 0.5 min). Optical fiber tip was directed perpendicular to the tissue surface. Anesthesia of rats was carried out with a solution zoletil. Zoletil was administered intraperitoneally at a dose of 80 mg/kg. The rats were decapitated after 1 hour of exposure. To determine the changes in LP the standard colorimetric test OXYSTAT was used.
The results indicate an increase in the intensity of free radical generation in tissues induced by photodynamic treatment.

Representing author

photo

Irina Yur'evna Yanina

Saratov State University, scientific researcher
Saratov, Russia

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