Biophysics, Poster

THE DIGITAL MICROSCOPY TECHNIQUE TO MONITOR CREATION OF PORES IN FAT CELL’S MEMBRANE

I.Yu. Yanina1,2, A.V. Gordeev1, Sh.R. Dahchukov1, V.A. Doubrovsky,1 V.V. Tuchin2,3,4

1Saratov State Medical University (SSMU), Russia
2Saratov State University (SSU), Russia
3Institute of Precise Mechanics and Control RAS, Russia
4University of Oulu, Finland

ABSTRACT

By means of digital microscopy “specific structures” on the fat cells’ membranes were registered, which may be treated as cytoplasm/oil liquid drops flowed out through the pores in the membranes. It was shown experimentally that fat cells treatment by alcohol increases the quantity of “specific structures”, hence, pores in membrane, even more – the increase of alcohol concentration in water solution leads to the increase of quantity of pores. The influence of Brilliant Green dissolved in alcohol upon the quantity and space sizes of “specific structures” on the membranes was investigated. The experiments with Brilliant Green were fulfilled in two variants: in the regime of selective light irradiation of cells and without light. The experiments demonstrated that optical selective action on the fat cells sensitized by Brilliant Green leads to the growth of the number of “specific structures” (pores) during some time after the light is switched off. These results prove our early suggested mechanism of photobiological action of light on sensitized fat cells: formation of pores in membrane. The digital microscopy technique for imaging of cytoplasm/oil drops (clouds) flowed out through the cell membrane via pores can be considered as an optical technique to monitor existence of pores on nm scale.

Representing author

photo

Irina Yur'evna Yanina

Saratov State University, scientific researcher
Saratov, Russia

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