Biophotonics, Internet Report

A COMPARATIVE STUDY OF IMPACT OF X-RAY CONTRAST OMNIPAQUETM USED AS AN OPTICAL CLEARING AGENT ON BLOOD MICROCIRCULATION IN RATS WITH ALLOXAN DIABETES BY LASER SPECKLE CONTRAST IMAGING

Timoshina P.A.(1), Bucharskaya A.B.(2), Tuchin V.V.(1,3,4); 1 - Research-Education Institute of Optics and Biophotonics, Saratov National Research State University, Russia; 2- Saratov State Medical University, Russia; 3 - Institute of Precision Mechanics and Control, Russian Academy of Sciences, Saratov, Russia; 4 - Interdisciplinary Laboratory on Biophotonics, National Research Tomsk State University, Russia

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ABSTRACT

The study of blood microcirculation is one of the urgent problems of the medicine. Hemodynamic changes in diabetes contribute to the emergence of hypoxemia in various organs, leading to retinopathy, neuropathy, nephropathy, coronary heart disease, and peripheral arterial disease. We present the results of experimental study of changes of microcirculation of blood flow of pancreas in rats with diabetes under the influence of optical clearing agents by using Laser Speckle Contrast Imaging (LSCI). Laser speckle contrast techniques are based on the spatial and temporal statistics of the speckle pattern, calculating of contrast of time-averaged dynamic speckles in dependence on the exposure time at the registration of the speckle-modulated images. The influence of 70% aqueous solution of x-ray contrast OmnipaqueTM 300 (n = 1.407) and 100% OmnipaqueTM 300 (n = 1.438) was investigated. In our research, we used 40 white laboratory rats weighing 300-500 g. We used alloxan induced animal model of diabetes. Results show that in pancreas of diabetic rats group blood velocity increased relative to the control group. Topical application of 70% and 100% OmnipaqueTM 300 showed change of blood velocity in the blood vessels of diabetic group of laboratory animals, and the blood flow in the control group did not change. The results obtained at the study of blood microcirculation disorders of pancreas in diabetes show that disease development in animals causes changes in the microcirculatory system.

Representing author

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Ms. Polina Aleksandrovna Dyachenko /Timoshina/

Saratov State University, PhD, Engineer, Assistant
Saratov, Russia

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