Daria K. Tuchina,1 Yu. P. Razdelkina,1 Alexey N. Bashkatov,1,2, Elina A. Genina,1,2 Valery V. Tuchin1,2,3
1Research-Education Institute of Optics and Biophotonics, Saratov National Research State University, Saratov, Russia
2Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, Tomsk, Russia
3Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control RAS, Saratov, 410028, Russia
Glycerol solutions can be applied for reduction of tissue scattering and consequently increase of light penetration depth in tissue (tissue optical clearing). Since optical methods of diagnostics and treatment are widely used in medicine and cosmetology, tissue optical clearing contribute to effective application of light to tissue measurements and control. Diffusion rate of glycerol determines how fast optical clearing will be achieved.
The goal of the study was to quantify glycerol diffusion in ex vivo rat skin using optical method.
In this study, skin samples were obtained by autopsy from white rats. The collimated transmittance kinetics of skin sample was measured in the spectral range of 500-900 nm using USB4000-Vis-NIR spectrometer (Ocean Optics, USA) concurrently with administration of the aqueous 40%-glycerol solution (refractive index n = 1.384). Each sample was fixed in the cuvette filled up with glycerol solution using the plastic plate with a square aperture of 7×7 mm2 in the center.
The increase of collimated transmittance and its further saturation was observed for all skin samples during its immersion in 40%-glycerol solution. Collimated transmittance kinetics was used to quantify glycerol diffusion skin ex vivo. The glycerol diffusion coefficient in rat skin was found as (2.17±0.76)*10^-6 cm2/s.
Results obtained in the study can be used for evaluation of tissue permeability for prediction and reduction of light attenuation in skin at disease diagnostics and treatment.
Daria Kirillovna Tuchina
Saratov National Research State University, Assistant Professor, Engineer
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