Biophysics, Poster

SPIN PROBE AS A POSSIBLE DIAGNOSTIC TOOL

Eugenia Nemova, Institute of Laser Physics SB RAS, Russia

ABSTRACT

It is demonstrated in [1] that the terahertz radiation can act as a provoking factor allowing one to reveal the liability to the insufficiency of the immune system and to hematological diseases in practically healthy persons. In this work, the possibility of the effect of terahertz radiation on the paramagnetic properties of the whole blood from patients with normal and increased level of leukocytes was investigated. The changes in the oxidative status of blood, accompanying the conformational changes, can be an important aspect for diagnostics. Spin labeling and/or spin probing are among the informative methods to reveal such conformational changes. To follow the conformational rearrangements of proteins, spin labels are usually chemically bound to definite sites of a protein molecule. In our work, we used dihydropyrazone-1,4-dioxide (DPDO) as a precursor of the spin probe formed in situ. This compound is a cyclic dinitrone known as spin trap [2]. The EPR spectrum of the whole blood with the probe is a triplet characterized by the hyperfine splitting constant (hfc)aN =14,96 G and line width l = 7,36 G. It was discovered that the major parameters of the EPR spectra recorded with the blood with normal leukocyte content remain almost unchanged after irradiation within the terahertz range, while the changes in the EPR spectra of blood with increased leukocyte content are clearly pronounced. Measurement of the width of the first (low-field) component in the EPR spectrum showed that the line width slightly decreases as a result of irradiation of the blood with normal leukocyte content in comparison with non-irradiated blood, (from 8.62 to 8.45 G), while it increases as a result of irradiation of the blood with increased leukocyte content (from 7.22 to 8.04 G). This allows us to expect that the terahertz irradiation of the samples of the whole blood can be helpful in determining the transient states leading to leukocytosis, which can be an essential marker of various pathologies.

References
1. Fedorov V. I., Klementyev V. M., Khamoyan A. G., Shevela E. Ya., Chernykh E. R. Submillimeter laser as a potential tool for medical diagnostics //
Millimetrovye volny v biologii I meditsine (Millimeter waves in biology and medicine). 2009, №1-2(53-54). p. 88-97 (in Russian).
2. Dultseva G.G., Skubnevskaya G.I., Tikhonov A.Ya et al. // J. Phys. Chem., 1996, v. 100, p. 17523–17527.

Representing author

photo

Ms. Eugenia Fedorovna Nemova

Institute of Laser Physics SB RAS, ~
Novosibirsk, Russia

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