Biophotonics, Internet Report


Daria K.Tuchina1, Alexey N. Bashkatov1,2, Polina A. Timoshina1, Elina A. Genina1,2, Valery V. Tuchin1-3
1Research-Educational Institute of Optics and Biophotonics, Saratov State University, Saratov, Russia
2Interdisciplinary Laboratory of Biophotonics, Tomsk State University, Tomsk, Russia
3Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precise Mechanics and Control RAS, Saratov, Russia

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The goal of this study was investigation of 40%-glucose solution impact on skin optical properties and morphology. Twenty samples of rat skin were used to obtain weight, area and thickness kinetics under action of 40%-glucose solution. Ten samples were immersed in the solution, the weight and thickness of samples (ten samples) were measured using the electronic balance (SCIENTECH, SA210, USA) with the precision of 1 mg and a micrometer with a precision of 10 ┬Ám, respectively, before immersion and every 5 min during the immersion. Ten samples were used for analysis of digital images of samples for obtaining the samples area within a period of immersion. Transverse and along shrinkage and weight loss for 20-60 min of immersion; and subsequent transverse and along swelling and weight increase were observed.
Ten samples of rat skin were used to measure glucose diffusion coefficient in skin in vitro. Intact samples were placed in cuvette with 40%-glucose solution and temporal collimated transmittance spectra were measured using USB4000-Vis-NIR spectrometer (Ocean Optics, USA) in the spectral range 400-1000 nm. Increasing of collimated transmittance of the skin samples caused by refractive index matching of tissue scatterers and interstitial fluid was observed. Basing on these data the glucose diffusion coefficient in skin was calculated using the specially developed computer program.
Also we present the results of experimental study of 40%-glucose solution impact on microcirculation of blood flow of underlying tissues in rats in vivo with Laser Speckle Contrast Imaging (LSCI). In this research we used ten white laboratory rats weighing 200-300 g. As the results, 40%-glucose solution has a vasodilator effect, expressed in the increase of microvessel size and the decrease of average speed of microcirculation (increase in value of speckle contrast image).
The obtained results can be used for optimization of tissue optical clearing and drug delivery techniques, improvement of biophysical and mathematical models describing interactions between tissues and chemical agents.

Representing author


Daria Kirillovna Tuchina

Saratov National Research State University, Assistant Professor, Engineer
Saratov, Russia

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