Biophotonics, Internet Invited Lecture

TISSUE OPTICAL CLEARING FOR ENHANCEMENT OF IN VIVO BLOOD FLOW IMAGING

Dan Zhu, Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology, P.R.China

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ABSTRACT

The tissue optical clearing (TOC) technique could significantly improve the biomedical optical imaging depth, but most current investigations are limited to in vitro studies. For in vivo applications, the TOC method must provide a rapid treatment process, sufficient transparency, and safety for animals, which makes it more difficult. Recently developed innovative optical clearing methods for in vivo use show great potential for enhancing the contrast and resolution of blood flow imaging. This paper gives an overview of recent progress in the use of TOC for vascular visualization. First, this presentation introduces transparent windows, including various skin windows and a cranial window, that permit Laser speckle imaging to monitor dermal or cortical blood flow, respectively, with high resolution and contrast. We demonstrate the capability of the combination method of LSCI and skin optical clearing to describe in detail the dynamic response of cutaneous vasculature to vasoactive noradrenaline injection. Moreover, the superior resolution, contrast and sensitivity make it possible to rebuild arteries-veins separation and quantitatively assess the blood flow dynamical changes in terms of flow velocity and vascular diameter at single artery or vein level.

Representing author

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Dr. Dan Zhu

Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology, Professor
Wuhan, P.R.China

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