Biophotonics, Internet Invited Lecture

FLUORESCENCE SPECTROSCOPY AND MICROSCOPY OF CUTANEOUS TUMOURS – CORRELATION BETWEEN MICRO- AND MACRO- SPECTRAL MEASUREMENTS

Ekaterina Borisova, Latchezar Avramov
1) Institute of Electronics, Bulgarian Academy of Sciences, 72, Tsarigradsko chaussee Blvd., 1784 Sofia, Bulgaria, e-mail: borisova@ie.bas.bg
Maria Lomova, Oksana Semyachkina-Glushkovskaya, Dmitry Gorin
2) Saratov State University, Russia, Astrakhanskaya Str. 83, Saratov 410012, Russia

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ABSTRACT

Autofluorescence spectral and confocal microscopic measurements were made on different cutaneous neoplastic lesions, namely basal cell carcinoma, squamous cell carcinoma, malignant melanoma, and their dysplastic forms – keratoacantoma, dysplastic nevi and benign lesions related – basal cell papiloma, seboreic keratosa and compound nevi using excitation at 405 nm.
Spectroscopic investigations were made on in vivo and ex vivo tissue samples, and confocal microscopy investigations were made on ex vivo and haematoxilin stained thin slices of the tumours detected. Specific spectral features observed in each type of lesion investigated on micro and macro level would be presented and discussed. Correlation between the spectral data received and the microscopic features observed would be discussed in the report.
Acknowledgements: This work was supported in part by the Ministry of Education and Science of Russian Federation under Grant № 17.488.2014/K and by National Science Fund of Bulgarian Ministry of Education and Science under grant № DFNI-B02/9/2014.

Representing author

photo

Dr. Ekaterina Georgieva Borisova

Institute of Electronics, Bulgarian Academy of Sciences, Associate Professor
Sofia, Bulgaria

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