Biophysics, Poster

QANTUM DOTS AS LABELS IN NON-INSTRUMENTAL GEL-BASED IMMUNOASSAY

Goryacheva I.Yu., Speranskaya E.S., Beloglazova N.V. Saratov State University, Chemistry Institute

ABSTRACT

Quantum dots (QDs) are inorganic luminescent semiconductor nanocrystals, exhibited size-dependent fluorescence emission spectra. For the last years QDs application in immunoassay could be mentioned as the most growing area of immunoassay labeling. QDs were used as fluorescent labels for microtiter plate assay (fluorescence-linked immunosorbent assay FLISA) for the detection of analytes of different nature in various matrixes. QDs application as labels for non-instrumental tests were not published till 2009 (according to Web of Science). And in 2010 application of QDs as labels for lateral flow immunoassay was described as for small molecule, as for proteins.
We have developed new column gel-based immunoassays for the model analyte benzo[a]pyrene and compared three different kinds of labels, i.e., horseradish peroxidase, colloidal gold and QDs with respect to rapid visual on-site testing. It was shown, that the best sensitivity and the shortest assay procedure were obtained with QD label. For the model analyte (benzo[a]pyrene) limit of detection was at 5 ng L-1.

Natalia V. Beloglazova, Irina Yu. Goryacheva, Reinhard Niessner, Dietmar Knopp A comparison of horseradish peroxidase, gold nanoparticles and qantum dots as labels in non-instrumental gel-based immunoassay. Microchimica Acta in press. DOI: 10.1007/s00604-011-0682-4.

Representing author

photo

Dr. Irina Goryacheva

Saratov State University, Associate Professor
Saratov, Russia

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