Biophotonics, Internet Report

BLOOD MICROCIRCULATION IN MOUSE BRAIN WITH ALLOXAN DIABETES STUDIED BY TEMPORAL LASER SPECKLE CONTRAST IMAGING

Timoshina Polina(1,2), Rui Shi(2), Yang Zhang(2), Dun Zhu(2) Tuchin Valery(1,2,3), Qingming Luo(2)
1- Research-Educational Institute of Optics and Biophotonics, Saratov State University, Russia
2- Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, China
3- Institute of Precision Mechanics and Control, Russian Academy of Sciences, Saratov, Russia

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ABSTRACT

The study of blood microcirculation is one of the most important problems of the medicine. This is caused by the fact that many diseases, such as cardio-vascular diseases, atherosclerosis, diabetes, chronic venous insufficiency cause functional and morphological changes of microcirculation of blood flow. We present the results of experimental study of changes of microcirculation of blood flow of brain in mice with diabetes by using Temporal Laser Speckle Contrast Imaging (TLSCI). Additionally a direct effect of glucose water solution (concentration 20% and 40%) on blood microcirculation was studied. In our research we used 20 white laboratory mice weighing 20-30 g. We used alloxan induced animal model of diabetes. The TLSCI method is based on registration of temporal statistics, computing of speckle contrast images using a sequence of speckle images acquired along a few time points instead of using a spatial window. Results show that in brain of diabetic mouse group diameter of sagittal vein is increased and the speed of blood flow reduced relative to the control group. Topical application of 20%- or 40%-glucose solutions also causes increase of diameter of blood vessels and slows down blood microcirculation. The results obtained at the study of blood microcirculation disorders of brain in diabetes show that disease development in animals causes changes in the microcirculatory system.

Representing author

photo

Ms. Polina Aleksandrovna Dyachenko /Timoshina/

Saratov State University, PhD, Engineer, Assistant
Saratov, Russia

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