Biophotonics, Internet Report

EFFECT OF GLUCOSE SOLUTIONS ON THE OPTICAL AND STRUCTURAL PROPERTIES OF SKIN TISSUE

Daria Tuchina, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China; Research-Educational Institute of Optics and Biophotonics, Saratov State University named after N.G. Chernyshevsky, Russia
Rui Shi, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China
Alexey N. Bashkatov Research-Educational Institute of Optics and Biophotonics, Saratov State University named after N.G. Chernyshevsky, Russia
Elina A. Genina Research-Educational Institute of Optics and Biophotonics, Saratov State University named after N.G. Chernyshevsky, Russia
Dan Zhu Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China
Qingming Luo Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China
Valery V. Tuchin Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China; Research-Educational Institute of Optics and Biophotonics, Saratov State University, Saratov, Russia; Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precise Mechanics and Control RAS, Saratov, Russia; Optoelectronics and Measurement Techniques Laboratory, University of Oulu, Oulu, Finland

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ABSTRACT

In this study we present experimental results of in vitro investigation of optical clearing of skin tissue by glucose solutions with different concentrations. 60 samples of mouse skin tissue have been used in this study. The collimated transmittance kinetics of 30 skin samples were measured using USB4000-Vis-NIR spectrometer (Ocean Optics, USA) concurrently with administration of the 30%-, 43%- and 56%-glucose solutions in the spectral range 400-1000 nm.
30 skin samples have been used for investigation of influence of the glucose solutions on the structural parameters of the mouse skin. All samples have been immersed in different cuvettes with the glucose solutions and their weight, thickness and area have been measured consequently each 5 min. Thickness of the samples was measured using confocal microscope (FV 1000, Olympus, Japan). Weight of the samples was measured during action of glucose solutions using weight balance (PL203, Mettler Toledo, China) with the precision of 1 mg. Area of each sample was measured by processing of digital images of the samples. The measurements were performed at room temperature about 20°C.
As a result, we observed the increase of collimated transmittance in the first 2-3 min of the immersion and its further saturation. The decrease of weight, thickness and area (the tissue shrinkage) was observed.
The obtained results can be used for optimization of existing optical clearing technologies, improving of the mathematic models describing interaction between optical clearing agent and tissues and for determination of structural changes and tissue permeability in pathological conditions.

Representing author

photo

Daria Kirillovna Tuchina

Saratov National Research State University, Assistant Professor, Engineer
Saratov, Russia

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