Biophysics, Oral Report

EXPERIMENTAL MODEL FOR FLUORESCENT IMAGING OF TUMOR GROWTH AND REGRESSION UNDER THERAPEUTIC TREATMENT

E.A. Malekhanova
N.I. Lobachevsky State University of Nizhny Novgorod
N.Y. Lekanova
N.I. Lobachevsky State University of Nizhny Novgorod
I.V. Krutova
N.I. Lobachevsky State University of Nizhny Novgorod
I.V. Balalaeva
N.I. Lobachevsky State University of Nizhny Novgorod
S.M. Deyev
Institute of Bioorganic Chemistry of Russian Academy of Sciences

ABSTRACT

Fluorescent agents used for cells and molecules visualization are known to be a powerful tool for study of different processes in living systems. Fluorescent proteins have recently been used as fluorescent probes. Unique properties of these genetic markers (stable fluorescence, cofactor independence, nontoxicity) make them a successful alternative of organic dyes. One of the most perspective fields for fluorescent proteins application is considered to be experimental oncology.
The goals of this research were, firstly, to approbate a fluorescent tumor model for estimation of therapeutic efficiency, and, secondly, to compare information value of two methods – fluorescent bioimaging and standard tumor size measuring technique.
The experiment was carried out on nude female mice with subcutaneous transplanted human ovary carcinoma, transfected with fluorescent protein Katushka (SKOV-3-kat cell line). As the therapeutic agents we used the standard chemotherapy drug cisplatin and the immunotoxin 4D5-ExoA, designed on the basis of scFv-antibody to HER-2/neu receptor. Two-dimensional fluorescent images were obtained in vivo with the fluorescent imaging setup (Institute of Applied Physics RAS).
Tumor growth monitoring by fluorescent imaging and standard technique (external caliper measurement) has found out a considerable correlation between tumor volume increase and integral fluorescence changes. Moreover, it is shown that fluorescent method provides additional information about cells allocation in tumor node volume and allows nonpalpable tumor visualization, that gives an opportunity to estimate an action of therapy on early stages. In addition, apparent antitumor effect of the immunotoxin 4D5-ExoA was revealed, which is comparable with the standard cisplatin treatment.
Thus, our results confirm that application of the red fluorescent proteins for non-invasive tumor growth study and therapeutic efficiency research is promising.

Representing author

photo

Evgeniya Malekhanova

Nizhny Novgorod State University
Russia

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